Author Archives: Jeff

Roadmap to Ocean Worlds: Polar microbial ecology and the search for totally normal life

Recently congress recommended that NASA create an Ocean Worlds Exploration Program whose primary goal is “to discover extant life on another world using a mix of Discovery, New Frontiers, and flagship class missions”.  Pretty awesome.  In February I was invited … Continue reading

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Phylogenetic placement re-re-visited

I use phylogenetic placement, namely the program pplacer, in a lot of my publications.  It is also a core part of of the paprica metabolic inference pipeline.  As a result I field a lot questions from people trying to integrate … Continue reading

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Another victim of science funding

Or rather the lack thereof.  I was very disappointed to receive an email yesterday that BioCyc, a popular database of enzymes and metabolic pathways in model organisms, is moving to a subscription model.  The email is posted below in its … Continue reading

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Tutorial: Annotating metagenomes with paprica-mg

This tutorial is both a work in progress and a living document.  If you see an error, or want something added, please let me know by leaving a comment. Starting with version 3.0.0 paprica contains a metagenomic annotation module.  This … Continue reading

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Antarctic wind-driven bacterial dispersal paper published

I’m happy to report that one of the appendices in my dissertation was just published in the journal Polar Biology.  The paper, titled Wind-driven distribution of bacteria in coastal Antarctica: evidence from the Ross Sea region, was a long time … Continue reading

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Tutorial: Building the paprica database

This tutorial is both a work in progress and a living document.  If you see an error, or want something added, please let me know by leaving a comment. Building the paprica database provides maximum flexibility but involves more moving … Continue reading

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Correctly evaluating metabolic inference methods

Last week I gave a talk at the biennial Ocean Sciences Meeting that included some results from analysis with paprica.  Since paprica is a relatively new method I showed the below figure to demonstrate that paprica works.  The figure shows … Continue reading

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Tutorial: Analysis with paprica

This tutorial is both a work in progress and a living document.  If you see an error, or want something added, please let me know by leaving a comment. Getting Started I’ve been making a lot of improvements to paprica, … Continue reading

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Tutorial: Installing paprica on Mac OSX

The following is a paprica installation tutorial for novice users on Mac OSX (installation on Linux is quite a bit simpler). If you’re comfortable editing your PATH and installing things using bash you probably don’t need to follow this tutorial, … Continue reading

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Wrapping up the season

Yesterday morning the Gould returned to Palmer Station, which means that it’s time for Jamie and I to take off. I’m looking forward to getting home and working through all the data we’ve collected (and who wouldn’t want to spend … Continue reading

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